However, the intracellular MFI in the c-fos WT group was approximately 60% lower than that for the NC group (p?

However, the intracellular MFI in the c-fos WT group was approximately 60% lower than that for the NC group (p?Klf2 Conclusion C-fos increased the expression of P-gp and mdr1 in the HEp-2/VCR cells, and enhanced the efflux function of the cells, thereby contributing to the development of MDR. values less than 0.05 were considered statistically significant. Results Drug resistance of HEp-2/VCR cells We established a drug-resistant human laryngeal carcinoma cell line, named HEp-2/VCR, by selection against an increasing drug concentration gradient. The IC50 of VCR was increased from 0.04??0.01?mol/l in the normal HEp-2 cells to 1 1.7??0.19?mol/l in the HEp-2/VCR cells (Table?2). The 42.5-fold increase in IC50 indicates successful establishment of the drug-resistant HEp-2/VCR cell line. Table 2 Comparison of the IC50 values for HEp-2 and HEp-2/VCR cells exposed to 4 chemotherapeutics

IC50/(mol/l) Anti-cancer drugs Resistant fold HEp-2 HEp-2/VCR

VCR0.04??0.011.7??0.1942.5MTX1.2??0.358.3??0.236.90DDP0.5??0.251.9??0.163.85-FU61.1??4.35332??5.215.44 Open in a separate window Data are shown as the means SD The IC50 values for other common chemotherapeutic drugs were also assessed (Table?2). HEp-2/VCR cells were respectively 6.90, 3.8 and 5.44 times as resistant as HEp-2 cells to MTX, DDP and 5-FU. The results indicate that HEp-2/VCR is a multidrug-resistant cell line. Expression of c-fos and mdr1 in HEp-2/VCR cells Real-time PCR results showed that the expression of the proto-oncogene c-fos was low in HEp-2 cells, but increased 4.66-fold in the drug-resistant HEp-2/VCR cells (p?Finafloxacin hydrochloride HEp-2 and HEp-2/VCR cells. c, d Western blot analysis of the expression of c-fos and p-gp. e, f The statistical quantification analyses of c-fos and p-gp protein levels in Finafloxacin hydrochloride HEp-2 and HEp-2/VCR cells. Data are shown as the means SD.*p?p?p?

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